The bisy expression vector toolbox includes vectors with constitutive, methanol-inducible or de-repressible (methanol - independent) promotors. Vectors are available as integrative or episomal expression system as well as for intracellular or secreted production of target proteins. For the efficient secretion of target proteins different secretions signals are applied. Beside various antibiotic selection markers, auxotrophic markers for antibiotic independent selection are in the portfolio.

bisy GmbHs proficiency in the production of heterologous proteins is built on more than 20 years of experience. We offer customized solutions for Pichia pastoris / Komagataella phaffii protein expression for all kinds of proteins including enzymes, structural and processing proteins, biopharmaceuticals and biocatalysts. Codon sequence optimization for our expression host Pichia pastoris is our daily business as well as improving protein properties by directed evolution and rational protein design.

Choosing the right strain and expression strategy for recombinant proteins can be challenging. Bisy offers a range of Komagataella phaffii platform strains tailored to diverse applications. With 20+ years of expertise in strain engineering, we also develop customized strains and vectors. Our portfolio includes methanol-dependent and methanol-free expression systems, diverse secretion signals, bidirectional expression for titer enhancement, co-expression solutions, and extrachromosomal plasmids for library screenings.

We guide clients from feasibility studies to commercial-scale production. By progressively scaling from microtiter plates and shake flasks to pilot trials, we generate data that helps assess product potential and address challenges early. Beyond technical solutions, we prioritize timelines, cost control, legal considerations, and economic efficiency. Each project begins with a detailed needs analysis, leading to a milestone-based approach for transparency and effective project management. The initial projects usually create the basis for long term collaborations.

Streamline your development journey with our bioinformatics expertise, from early stages to EFSA/FDA approval. Leverage our in-house Illumina Whole Genome (re)Sequencing (WGS) pipeline or opt for de novo assembly with advanced long-read technologies. Our RNA-seq analysis helps identify bottlenecks in cultivation and fermentation, ensuring high-quality platform strains through manually curated genome annotations. Our standalone bioinformatics services provide species and strain identification via phylogenetic and phylogenomic analysis. Detect transposable elements, antibiotic resistance genes, and metabolic clusters with cutting-edge analytical tools.

Bisy’s organic lab is entirely separate from our main biotech facility, ensuring a GMO-free environment for natural fermentation and food innovation. With a small-scale brewery and expertise in enzymatic food processing, we are able to develop high-quality, natural products under strict organic standards, free from genetic modification.

For practitioners and researchers who want to expand their expertise on the Pichia pastoris (Komagataella phaffii) expression and production system, bisy GmbH offers individualized scientific trainings. In practical and theoretical sessions you can intensify your knowledge ranging from optimization of expression strategies and state-of-the-art laboratory procedures to bioprocess design and data evaluation.